For ABPP analyses only, outlier samples (based on low precursor identifications or clustering by principle coordinate analysis) were removed, leaving a minimum of 3/4 samples per condition
The GSH/GSSG perturbations in severe asthma were further associated with cysteine depletion and increased oxidation of the cysteine/CySS redox potential in the epithelial lining fluid, suggestive of global airway thiol imbalance and possibly decreased cysteine reserves for additional GSH synthesis (86)
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In the wing injury assay, we used the axotomy model developed by Fang et al (2012) and Neukomm et al (2014)
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However, we controlled for CPZ equivalents, which did not correlate with gFA or HV in patients