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Materals and methods Materials MTX was obtained from David Bull Laboratories, Mulgrave-Victoria, Australia

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Cells were prepared and stained as per the Leica protocol ( In brief, cells were plated onto coverslips and treated with or without 5FU at the indicated doses for 72 h

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When the antioxidant glutathione is administered intravenously, this causes an immediate boost in energy since the antioxidant is known to enhance mitochondrial energy production in cells