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380 The Ni/Co specificity of InrS over Cu I and Zn II has been evaluated both in vivo and In vitro , revealing that the affinity of InrS for these other transition metal ions is sufficiently low, i.e., an insufficiently negative G relative to Ni II (Figure 8), which prevents induction of the expression of genes encoding Ni II transporters as a result of mismetalation (Figure 12D)