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Pulsed Doppler interrogation of mitral inflow was performed to measure peak early diastolic velocity (E), peak late diastolic velocity (A), their ratio E/A, E wave deceleration time, and isovolumic relaxation time

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After cultivation, supernatants of PCLS cultures were removed and 250 L of 1:10 WST-1 reagent dilution was added

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That is one of the reasons his work has remained discussable across decades