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Similarly, exposure of primary normal human bronchial epithelial (NHBE) cells to two commonly used flavouring chemicals (diacetyl or 2,3-pentanedione) for 24 h evoked differential expression of 163 and 568 genes respectively [32], while a cytotoxic effect on mouse neuronal cells has also been reported [59]

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Based on the -SH groups capacity to reduce 5, 50-dithiobis, 2-nitrobenzoic acid (DTNB) and produce a yellow-colored anionic product, the optical density of which was measured at 412 nm, both evaluations were made