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Nicotine equivalency was determined by quantifying serum cotinine levels (a nicotine metabolite) in blood samples collected 30 min after exposure
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The cigarette of present embodiment adopts the cigarette of certain brand, and filter tip is 3 centimeters long, and diameter is 8 millimeters, and cigarette filter tip part is cut off, and the amount fraction of immersing substance is 6% pyruvic acid solution, 6% sodium pyruvate respectively solution, 6% magnesium pyruvate solution, and 6% calcium pyruvate solution were taken out and vacuum-dried to constant weight at room temperature
