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The samples were then cooled, diluted with 1 mL of distilled water and optical density was measured at 535 nm in a Genesys-20 (Thermo Scientific, USA) spectrophotometer

People need to contact a doctor if they experience: Persistent coughing: This involves a cough that lasts more than 3 weeks or does not improve with home treatments

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It is important to visit a medical clinic and consult a primary care physician for guidance and to discuss the suitability of these medications