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Briefly, 15 l of plasma was transferred to a 1.5 mL microcentrifuge tube followed by addition of dH 2 O (200 L, 0.05% formic acid [FA]) containing cotinine- d 3 (250 nM), followed by dilution with ammonium acetate (10 mM, 200 L)

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After the role, he starred on PBS critically acclaimed Square One TV, a children's mathematics show, and then played Leon on HBOs Deadwood