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Briefly, cells grown on 12 mm glass coverslips (Matsunami Glass Industry, Osaka, Japan, Cat# 1-S) coated with 0.2% gelatin from porcine skin (Sigma Aldrich, Cat# G2500) were incubated with 75 nM LysoTracker Red for 30 min at 37 C in the dark

Do not forget to copy the solid and dotted lines inside the template

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Insert the ordinary cigarettes that finished the filling channel into the bottom left hole, and the filling rod smoothly slides the fragrant beads into the filling channel