By 1968, the Smoking Man had progressed enough in the hierarchy to push himself for the assassination of Martin Luther King Jr., concerned that King was "talking like a Maoist" and a threat to the American war effort in Vietnam
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Cells were first permeabilized using 0.1% Triton X-100 (Sigma-Aldrich, Germany) dissolved in PBS for 15 min at RT and then blocking buffer (2% BSA in sterile PBS) was added