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Cell pellets resuspended in BEGM + 0.5 PSA were filtered using a 70 m cell strainer, collected by centrifugation (5 min, 225 g , 4 C) and cryopreserved in freeze media (F-media, 30% FBS, 10% DMSO)

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John Dunne, the head of the UK Vaping Industry Association (UKVIA), says illegal vapes may cause damage to peoples health as they have not gone through the same testing regime as legal devices