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It should be noted that tobacco use continues to be the worlds leading preventable cause of death, killing approximately 6 million people annually [10]

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After 12 weeks of HFD and nicotine administration, the mice of the NLRP3 shRNA group displayed decreased atherosclerotic lesion size and less lipid deposition in aortic root compared with the mice without NLRP3 shRNA administration and the mice administrated with lenti-scramble (Supplementary Figure 2)