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A final inoculum of 100 L of adjusted bacterial cultures was added to 10 mL of refrigerated BHI media (1% v/v)
PMID 30860933.
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A 2025 study published in JAMA Network Open, which involved surveys of more than 10,000 high schoolers across the country, found that nearly twice as many teens used nicotine pouches in 2024 compared to 2023
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