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After hybridization, the arrays were washed and stained on a Fluidics Station FS450 using Affymetrix GeneChip Command Console Software (AGCC software version 3.2) with protocol FS450_0004 for Nugen Protocol (blood samples) and FS450_0001 for the IVT express protocol (tissue samples), then scanned using the GeneChip Scanner 3000 7G