Regular dental check-ups and proper oral hygiene practices are crucial for tobacco chewers to maintain good oral health
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Accordingly, the measurement and steady control of those parameters is a critical element in the high-speed production environment of the tobacco and cigarette industry, dealing with complex shapes, various products and variable materials
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The protein was blended with 2SDS loading buffer (1:1), incubated at 100C for 4 min, then electrophoresed in 12% SDS-polyacrylamide gel and transferred to polyvinylidene difluoride microporous membrane (Millipore, Massachusetts, USA) by electrophoresis
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