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The purpose of evaluation of sperm motility was to determine the percentage of motile sperm and sperm rate

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After tissue fixation and paraffin embedding, 5 m sections were incubated with anti-E-cadherin, ZO-1, Keap1 and Nrf2 antibodies (1:100 dilution) at 4 overnight followed by incubation with secondary antibody, at last stained with DAB and counterstained with hematoxylin