A stock solution of methylglyoxal (Sigma-Aldrich, M0252) was prepared at 0.1 mg/mL (1.37 mM) in culture medium, and a 3-fold serial dilution was likewise performed across six concentrations
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Many of the freezing mixtures which are to be found described in books are incorrectly so named, for although they themselves are below the freezing point, yet they are not sufficiently powerful to freeze any quantity of water, or other substances, when placed in a vessel within them
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