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Sample collection and storage Whole blood (450mL) was collected in triple polyvinyl chloride bags (JP Farma , Ribeiro Preto, Brazil) containing citrate phosphate dextrose adenine (CPDA-1) anticoagulant solution, centrifuged at 24 C and 2,535 rpm for 5 min (KR 4i, Thermo Scientific , USA), and its components separated by an automatic extractor (Giotto, Delcon , Italy)

Its revenues even go to prop up North Korean nukes