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With MRI and PET brain scans, scientists could see where and how much of the enzyme was in the brain

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2.5 Nicotine quantification by LC-MS Liquid chromatography was conducted before mass spectrometric analyses using an Agilent HPLC System Series 1100 (Agilent Technologies, Waldbronn, Germany) connected to a HCT Ultra PTM Discovery System (Bruker Daltonics, Bremen, Germany) with electrospray ionisation (ESI) and ion trapping

Leo I is famous for meeting with the feared Attila the Hun and persuading him to turn back his invading force that was threatening to overrun Western Europe